Nephroprotective effects of Lippia sidoides ethanolic extract against ischemia/reperfusion-induced acute kidney injury

Ischemia/reperfusion injury (I/R) is commonly related to acute kidney (AKI) and oxidative stress. Antioxidant agents are used treat this condition. Lippia sidoides a brazillian shrub with anti-inflammatory anti-oxidative properties. Thus, the aim of study evaluate effect ethanolic extract (LSEE) on in vivo vitro models AKI induced by I/R. Male Wistar rats were submitted unilateral nephrectomy ischemia contralateral for 60 min via clamping followed reperfusion 48 h. They divided into four groups: Sham, LSEE (sham-operated pre-treated LSEE), I/R (rats ischemia) I/R-LSEE treated before ischemia). Kidney tissues homogenates determine stress parameters nephrin expression. Plasma urine samples collected biochemical analysis. assays evaluated 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) flow cytometry Rhesus Monkey Epithelial Cells (LLC-MK2). The treatment prevented expression alterations, as well parameters. In assay, protected against cell death, reduced reactive oxygen species increased mitochondrial transmembrane potential. showed biotechnological potential new phytomedicine nephroprotective agent.